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Viruses are a kind of microbes with small size and no cell morphology. They have a simple structure and contain only one nucleic acid, with a coat of protein or lipoprotein, so they can be regarded as "a package of genes". The virus itself cannot grow and divide, and can only replicate a large number of progeny viruses in a certain host cell. After leaving the host cell, the virus cannot replicate and quickly loses its activity; compared with other microorganisms, the virus is not sensitive to antibiotics.
| RNA Viruses |
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| DNA Viruses |
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Viruses are one of the most contagious pathogens, killing millions of people every year, and the still-spreading COVID-19 epidemic has posed a huge threat to the health and socio-economic development of all human beings. Since most viruses do not have clinically effective drugs, rapid and accurate diagnosis at the early stage of viral infection is essential to prevent the rapid spread of pathogens.
Traditional Immunological Detection Methods
Advanced Immunological Detection Methods
| Nucleic Acid Molecular Hybridization Detection Method | Polymerase Chain Reaction Technology | |
|---|---|---|
| The original nucleic acid single strand with certain homology is hybridized under certain conditions according to the principle of complementary base pairing, and the two sides of the hybridization are the probe sequence and the nucleic acid sequence to be detected. The biggest advantage of this method is its strong specificity and high sensitivity. | Real-time fluorescence quantitative PCR technology (RT-PCR technology), adding fluorescent groups (fluorescent dyes or fluorescent probes) to the PCR reaction system, using the accumulation of fluorescent signals to detect the entire PCR process in real time, and finally perform quantitative analysis. The advantages of this method are: strong sensitivity and specificity, can detect a large number of samples, can detect viral nucleic acid sequences integrated with the host cell genome, and can also be used to detect viruses that are difficult to isolate. |
New methods and technologies such as anti-alkaline phosphatase bridging enzyme labeling method, gene chip technology, protein chip technology and suspension array technology are also increasingly applied to the detection and diagnosis of viruses.
In addition, the biosensing technology based on the CRISPR-Cas system can also be used for viral nucleic acid detection due to its high specificity and high sensitivity.
Utilizing the DeteCasTM platform based on the CRISPR-Cas system, CD BioSciences can provide various services in virus detection, such as DNA detection service and RNA detection service. If you are interested in these services, please contact us.
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