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Metal ions are essential trace elements in life processes such as gene synthesis, protein expression, and immune regulation. With the continuous acceleration of human industrialization, more and more metal ions are discharged into the environment, and they are generally not degradable and are continuously enriched with the food chain, thus leading to more and more serious metal ion pollution in the environment. Therefore, the rapid and efficient qualitative and quantitative detection of metal ions has become a hot spot of concern at present.
The detection of metal ions is crucial for cell analysis and environmental monitoring. As a professional analyte detection company, CD BioSciences is committed to providing efficient metal ion detection service.
The CRISPR-Cas system is derived from the adaptive immune system of microorganisms such as bacteria, archaea and fungi, and consists of a combination of guide RNA and Cas enzymes. As an emerging technology in the field of biomolecules, it can be used as both a gene editing tool for precise manipulation and a sensitive and efficient biosensing tool. In the field of bioanalysis, the CRISPR-Cas system has the advantage of precise localization and efficient cleavage of target or non-target nucleic acids, and thus can be used to detect metal ions in the environment for contaminant monitoring.
A highly sensitive metal ion detection method combining DNAase and CRISPR system was developed, in which CRISPR-Cas12a system was used as an enhancer for DNA enzyme-based metal ion detection. This method is more sensitive than using DNAase detection alone, and it can be used for metal ion detection in ecological environments as well as in living cells.
When the metal ion is recognized in the detection system, the DNAase substrate strand is cleaved to produce single-stranded DNA. the resulting single-stranded DNA can be detected by the Cas protein/guide RNA complex, which in turn triggers the side strand cleavage effect of the CRISPR system, which can shorten the single-stranded DNA reporter indiscriminately and produce intense fluorescence. The presence or absence of metal ions can be determined by detecting the fluorescence signal.
Overview of Metal Ion Detection Principle (Yanju Chen et al, Analytica Chimica Acta, 2021)
Utilizing the CRISPR-Cas system's ability to specifically identify and cleave nucleic acid sequences, CD BioSciences offer highly sensitive metal ions detection service. In addition, we also provide detection services for nucleic acids, protein biomarkers, and small molecules.
Our DeteCas™ platform can detect metal ions from different environments, including but not limited to the followings.
Living Cell
Ecological Environment
Food
Cosmetics
CD BioSciences provides affordable, qualified and hassle-free metal ion detection service to our global clients. We guarantee on-time delivery of results. We are available to you 24 hours a day, so please feel free to contact us.
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