Nucleic Acids Detection Service

The detection of nucleic acids is vital for disease diagnosis, environmental and food safety monitoring. As a professional analyte detection company, CD BioSciences is committed to providing efficient nucleic acids detection service.

The CRISPR-Cas system is an adaptive immune defense system developed by bacteria and archaea during biological evolution for the removal of exogenous viruses or plasmid nucleic acids. Depending on the components, the CRISPR-Cas system can be classified as type I, type II, type III, type IV, etc. It can be used not only for gene editing, but also shows great potential for nucleic acids detection, which is important in the fields of pathogen detection, disease diagnosis, food and environmental monitoring, and nucleic acid imaging.

Nucleic Acids Detection by CRIPSR-Cas System

When the target nucleic acid is present in the system to be detected, the Cas protein specifically recognizes the target sequence under the guidance of the guide RNA and forms a complex, which is then stimulated with the "trans-cutting activity" against the cut single-stranded nucleic acid, shredding the fluorescent labeled probe in the system and emitting a fluorescent signal to complete the detection process. The presence or absence of the target nucleic acid can be determined by detecting whether the system is luminescent.

CRISPR-Cas9	CRISPR-Cas12	CRISPR-Cas13
The CRISPR-Cas9 system has the ability to precisely identify and cleave specific DNA sequences, and the combination of this property and isothermal amplification technology has developed some molecular assays that enable highly sensitive nucleic acids detection.	The CRISPR-associated protein Cas12, a type V of the class II CRISPR system, is an RNA-directed nucleic acid endonuclease with incidental cleavage activity. It can specifically cleave target nucleic acids under the guidance of crRNA with an accompanying non-specific cleavage activity of ssDNA. Therefore, it can be used for a variety of nucleic acids assays.	Cas13 is an RNA-guided nucleic acid endonuclease that specifically cleaves single-stranded target nucleic acids under the guidance of crRNA and can remain active and cleave other non-target nucleic acid after completion of cleavage. This property can be used for the detection of nucleic acids in bioanalysis.

Our Services

Utilizing the CRISPR-Cas system's ability to specifically identify and cleave nucleic acid sequences, CD BioSciences offer highly sensitive nucleic acids detection service. In addition, we also provide detection services for protein biomarkers, metal ions and small molecules.

Our DeteCas™ platform can detect nucleic acids molecules in different species and cell compartments, including but not limited to the followings.

DNA Detection Targets

Microorganisms DNA
Human DNA
Plant DNA
Parasitic Worm DNA

RNA Detection Targets

Protein Synthesis RNA
Nucleic Acid RNA
Gene Regulatory RNA
Post-transcriptional Modification RNA

Advantages of Our Service

High sensitivity for detection of low concentration substances.
High detection efficiency using an optimized CRISPR-Cas system.
Different detection solutions to meet different research needs.
Regular reporting of experimental progress.

Process of Our Service

CD BioSciences provides affordable, qualified and hassle-free nucleic acids detection service to our global clients. We guarantee on-time delivery of results. We are available to you 24 hours a day, so please feel free to contact us.

References

Hatoum-Aslan, A. CRISPR methods for nucleic acid detection herald the future of molecular diagnostics. Clinical Chemistry. 2018, 12(64): 1681–1683.
Tina, Y.L., et al. Accelerated RNA detection using tandem CRISPR nucleases. Nature Chemical Biology. 2021, 17:982-988.

For research use only. Not intended for any clinical use.