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The detection of small molecule is vital for cellular metabolism, food and environmental safety monitoring. As a professional analyte detection company, CD BioSciences is committed to providing efficient small molecule detection service.
Small molecules are usually organic compounds with molecular weight less than 1000 Da. In the life sciences, almost every cell contains hundreds of different organic molecules, which play a key role in interspecies information exchange, cell metabolism, and regulation of transcriptional processes, and regulate signal transduction and gene transcription by acting on specific signaling pathways in metabolic pathways. In addition, many small molecules are also food and environmental contaminants. Therefore, the qualitative analysis and quantitative detection of small molecules are particularly important.
The CRISPR-Cas system is an adaptive immune defense system by bacteria and archaea. It not only can be used for gene editing, but also shows great potential for small molecule detection, which is important in areas such as food and environmental monitoring and pathogenic bacterial infections.
Based on the trans cleavage activity of CRISPR-Casl2a, an efficient small molecule detection system called CaT-SMelor (CRISPR-Cas12a and aTF-mediated small molecule detector) was established by coupling immobilized aTF (allosteric transcription factor) using a fluorescent reporter system.
CaT-Smelor uses protein immobilization technology to immobilize aTF with DNA binding activity on microcrystalline cellulose particles. When a target small molecule is present, the aTF undergoes a conformational change induced by the small molecule thereby reducing its DNA binding activity and freeing the DNA from the aTF. By sequence design, the free DNA molecules can activate the trans cleavage activity of CRISPR-Cas12a system, resulting in the cleavage of the single-stranded fluorescent probe and the generation of fluorescence.
Overview of Small Molecule Detection (Liang Mindong et al, Nature Communications, 2019)
Utilizing the CRISPR-Cas system's ability to specifically identify and cleave nucleic acid sequences, CD BioSciences offer highly sensitive small molecule detection service. In addition, we also provide detection services for nucleic acids, protein biomarkers and metal ions.
Our DeteCas™ platform can detect protein biomarkers for different sources, including but not limited to the followings.
Food Safety Detection Targets
Environmental Contaminant Detection Targets
Disease Biomarker Detection Targets
Gout
Gastric Tumor
Non-small Cell Lung Cancer
Non-alcoholic Steatohepatitis
CD BioSciences provides affordable, qualified and hassle-free small molecule detection service to our global clients. We guarantee on-time delivery of results. We are available to you 24 hours a day, so please feel free to contact us.
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