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Rapid detection of RNA is crucial for disease diagnosis, environmental and food safety monitoring. As a professional analyte detection company, CD BioSciences is committed to providing efficient RNA detection service.
As an important genetic material, the correct expression of RNA is essential for regulating cellular functions. In the past few years, the continuous development of CRISPR-Cas system has made it an effective RNA detection tool, which can be used not only for ultra-sensitive, multi-targeted, rapid, and single-base specific in vitro detection of disease-associated RNAs such as viral RNAs and miRNAs, but also enables portable determination of complex clinical samples, which is useful for Rapid diagnosis of diseases in remote areas is of great importance, and also has great potential for promoting human health development.
When the target RNA is present in the system to be tested, the Cas protein specifically recognizes the target sequence under the guidance of the guide RNA and forms a complex, which in turn is stimulated with "trans cleavage activity" against the cleaved single-stranded nucleic acid, shredding the fluorescent labeled probe and emitting a fluorescent signal. Finally, the presence of target RNA is determined by detecting whether the system is luminescent or not.
Overview of RNA Detection Principle (Tina Y. Liu et al, Nature Chemical Biology, 2021)
| CRISPR-Cas12 | CRISPR-Cas13 |
|---|---|
| The CRISPR-associated protein Cas12 is a type V of the class II CRISPR system, an RNA-guided nucleic acid endonuclease with incidental cleavage activity. It can specifically cleave dsDNA under the guidance of crRNA and is accompanied by a non-specific cleavage activity of ssDNA. After reverse transcription of RNA into DNA, it can be used for RNA detection. | Cas13 is an RNA-guided RNA nucleic acid endonuclease that specifically cleaves single-stranded target RNA under the guidance of crRNA and can remain active and cleave other non-target RNA after completion of cleavage. This property can be used for the detection of RNA in bioanalysis. |
Utilizing the CRISPR-Cas system's ability to specifically identify and cleave nucleic acid sequences, CD BioSciences offer highly sensitive RNA detection service. In addition, we also provide detection services for DNA, protein biomarkers, metal ions and small molecules.
Our DeteCas™ platform can detect RNA molecules that perform different functions, including but not limited to the followings.
Protein Synthesis RNA
Nucleic Acid RNA
Gene Regulatory RNA
Post-transcriptional Modification RNA
CD BioSciences provides affordable, qualified and hassle-free RNA detection services to our global clients. We guarantee on-time delivery of results. We are available to you 24 hours a day, so please feel free to contact us.
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