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Due to its unique advantages, the CRISPR-Cas system has been used to develop biosensors. CD BioSciences, a premier biotechnology company, is committed to providing you with protein biosensor development service based on the CRISPR-Cas system.
In recent years, with the development of science and technology, protein detection technology has become more and more important for medical diagnosis, drug development, ecological safety and other fields. Traditional immunoassay methods have low sensitivity and poor specificity. In this context, protein sensors are receiving more and more attention due to their high sensitivity, low cost and high stability. Due to the efficient signal amplification capability of CRISPR-Cas system, it can be applied to the development of biosensors for protein detection.
Currently, several protein biosensors based on CRISPR-Cas technology have been developed. For example, CLISA, a biosensing system based on CRISPR-Cas13a as a signal amplification strategy, and CARPID, a biosensing system that uses the RNA targeting and proximity markers of CRISPR-CasRx to recognize the binding proteins of specific long non-coding RNAs (lncRNAs) in cells. All of these sensing systems can be used to detect various proteins.
Utilizing the CRISPR-Cas system's ability to specifically identify and incidentally cleave nucleic acid sequences, CD BioSciences offers protein biosensor development service. In addition, we also provide services for the detection of various analytes such as nucleic acids, protein biomarkers, metal ions, and small molecules.
Our DeteCasTM platform offers different strategies in protein biosensor development service, including but not limited to the followings.
Design of dsDNA Containing T7 Promoter SequenceIn the presence of target proteins, T7 polymerase recognizes the transcribed promoter sequence and produces single-stranded RNA molecules that allow for the conversion of protein signals and nucleic acid signals. Therefore, we can design suitable promoter sequences for transcription for you. |
Selection of Different Signal Amplification StrategiesFor protein detection, we can offer you different signal amplification strategies based on the CRISPR-Cas12 system and CRISPR-Cas13 system, respectively, to choose from. |
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Design of DNA ActivatorThe role of DNA activators in CRISPR biosensors is to be recognized by Cas proteins and stimulate the trans-cleavage activity of Cas proteins. We can design efficient DNA activators for you. |
Selection of Cas Effector ProteinsHigh quality Cas effector proteins are the basis for establishing high performance CRISPR-Cas biosensing systems. We can screen Cas effector proteins with excellent recognition efficiency and shear activity for you and shorten the detection time. |
Cancer Protein Biomarkers
Neurological Diseases Protein Biomarkers
Cardiovascular Diseases Protein Biomarkers
Immunological Diseases Protein Biomarkers
Drug Development
Disease Diagnosis
Environmental Monitoring
• High Flexibility
Only the given gRNA sequence needs to be changed for different gene targets.
• High Specificity
Single-base resolution detection of biosensors is fully possible.
• High Sensitivity
Target analytes can be detected at concentrations as low as 10-18 mol/L.
CD BioSciences provides affordable, qualified and hassle-free protein biosensor development service to our global clients. We guarantee on-time delivery of results. We are available to you 24 hours a day, so please feel free to contact us.
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