Nucleic Acid Biosensor Development Service

Due to its unique advantages, the CRISPR-Cas system has been used to develop biosensors. CD BioSciences, a premier biotechnology company, is committed to providing you with nucleic acid biosensor development service based on the CRISPR-Cas system.

CRISPR-Cas System-Based Nucleic Acid Biosensors

With its precise recognition as well as incidental cleavage capability, the CRISPR-Cas system has shown promising applications in the field of biosensing. In recent years, by coupling with nucleic acid amplification technology, the CRISPR-Cas biosensing system has increasingly become a favorable tool for nucleic acid detection.

Principle of CRISPR-Cas System-Based Nucleic Acid Biosensor Development
The current novel nucleic acid biosensors based on the CRISPR-Cas system are developed based on the specific recognition of target nucleic acid sequences by nucleic acids or probes, which amplify and convert biological signals into easily detectable physicochemical signals, and by processing these physicochemical signals, qualitative or quantitative detection of target nucleic acids can be achieved.
Examples of CRISPR-Cas System-Based Nucleic Acid Biosensors
At present, a variety of nucleic acid biosensors based on the CRISPR-Cas system have been developed. For example, the DETECTR biosensor platform was developed to take advantage of Cas12a's ability to non-specifically shear ssDNA upon binding to target DNA. As well as SHERLOCK, a biosensing system that exploits the incidental shearing activity of Cas13a, has been developed and has been successfully applied to the detection of pathogens such as SARS-CoV-2, norovirus, and dengue virus.

Our Services

Utilizing the CRISPR-Cas system's ability to specifically identify and incidentally cleave nucleic acid sequences, CD BioSciences offers nucleic acid biosensor development service. In addition, we also provide services for the detection of various analytes such as nucleic acids, protein biomarkers, metal ions, and small molecules.

Our DeteCas^TM platform offers different strategies in nucleic acid biosensor development service, including but not limited to the followings.

Constant Temperature Amplification of Target Nucleic Acids

We offer different thermostatic amplification techniques for different target nucleic acids to ensure high sensitivity of the biosensor.

Design of Efficient Signal Reporter Molecules

We can provide to design efficient signal reporter molecules based on the shear preferences of different Cas effector proteins, which facilitates the sensitivity of the sensing system.

Selection of Cas Effector Proteins

High quality Cas effector proteins are the basis for establishing high performance CRISPR-Cas biosensing systems. We can screen Cas effector proteins with excellent recognition efficiency and shear activity for you and shorten the detection time.

Optimization of gRNA Design

The gRNA sequence characteristics and design location are critical factors in determining the specificity, loading, shear activity and stability of the CRISPR-Cas biosensing system. We can design different gRNA sequences for the gene targets of different nucleic acids to be tested to improve the detection specificity.

Targets of Biosensor Detection

DNA Detection Targets

Microorganisms DNA
Human DNA
Plant DNA, etc.

RNA Detection Targets

Protein Synthesis RNA
Nucleic Acid RNA
Gene Regulatory RNA, etc.

Applications

Pathogen Detection

Food Safety Detection

Environmental Monitoring

Process of Our Service

Advantages of Our Service

• High Flexibility

Only the given gRNA sequence needs to be changed for different gene targets.

• High Specificity

Single-base resolution detection of biosensors is fully possible.

• High Sensitivity

Target analytes can be detected at concentrations as low as 10^-18 mol/L.

CD BioSciences provides affordable, qualified and hassle-free nucleic acid biosensor development service to our global clients. We guarantee on-time delivery of results. We are available to you 24 hours a day, so please feel free to contact us.

References

Xu, W.J., et al. Pathogen detection based on CRISPR/Cas biosensing principal bacteria detection technology. Chinese Journal of Biological Engineering. 2021, 41(8): 8.
Xie, S.Y., et al. Advancing sensing tech
For research use only. Not intended for any clinical use.
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