Online Inquiry

Metal Ion Biosensor Development Service

Due to its unique advantages, the CRISPR-Cas system has been used to develop biosensors. CD BioSciences, a premier biotechnology company, is committed to providing you with metal ion biosensor development service based on the CRISPR-Cas system.

CRISPR-Cas System-Based Metal Ion Biosensor

Metal ions are essential trace elements in life processes, and they are also key factors in environmental pollution. Therefore, the rapid and efficient qualitative and quantitative detection of metal ions has become a hot topic of current interest. The CRISPR-Cas system is derived from the adaptive immune system of microorganisms such as bacteria, archaea and fungi. In the field of bioanalysis, the CRISPR-Cas system has the advantages of precise targeting and efficient cleavage of target or non-target nucleic acids, and thus can be used as an efficient biosensing tool for detecting metal ions.

CRISPR-Cas System-Based Metal Ion Biosensor

  • Principle of CRISPR-Cas System-Based Metal Ion Biosensor Development
    When a target metal ion is present, the functional nucleic acid specifically recognizes the target, which releases the activated DNA (activator) it is bound to. The released DNA activator activates the ssDNase activity of the Cas protein, which further nonspecifically cleaves ssDNA with fluorescent and fluorescent burst patterns at both ends, ultimately generating a fluorescent signal. Qualitative and quantitative analysis of the target is determined by detecting fluorescence. Based on this principle, efficient metal ion biosensors based on CRISPR-Cas system can be developed.
  • Examples of CRISPR-Cas System-Based Metal Ion Biosensors
    Several metal ion biosensing systems based on CRISPR-Cas technology have already been developed. For example, highly sensitive lead detection methods combining DNA enzymes and CRISPR systems, and biosensing tools for Na+ quantification by functional DNA-regulated CRISPR-Cas12a. These above methods can be used for the detection of metal ions in living cells and ecological environments.

Our Services

Utilizing the CRISPR-Cas system's ability to specifically identify and incidentally cleave nucleic acid sequences, CD BioSciences offers metal ion biosensor development service. In addition, we also provide services for the detection of various analytes such as nucleic acids, protein biomarkers, metal ions, and small molecules.

Our DeteCasTM platform offers different strategies in metal ion biosensor development service, including but not limited to the followings.

Design of Functional Nucleic Acids Functional nucleic acids can specifically recognize and bind target metal ions and convert non-nucleic acid signals to nucleic acid signals. We can design different functional nucleic acids for different detection targets, such as aptamers and DNA enzymes, as recognition elements in biosensors.
Optimization of gRNA Design The gRNA sequence characteristics and design location are critical factors in determining the specificity, loading, shear activity and stability of the CRISPR-Cas biosensing system. We can design different gRNA sequences for the gene targets of different nucleic acids to be tested to improve the detection specificity.
Selection of Different Signal Amplification Strategies For metal ion detection, we can offer you different signal amplification strategies based on the CRISPR-Cas12 system and CRISPR-Cas14 system, respectively, to choose from.
Selection of Cas Effector Proteins High quality Cas effector proteins are the basis for establishing high performance CRISPR-Cas biosensing systems. We can screen Cas effector proteins with excellent recognition efficiency and shear activity for you and shorten the detection time.
Design of DNA Activator The role of DNA activators in CRISPR biosensors is to be recognized by Cas proteins and stimulate the trans-cleavage activity of Cas proteins. We can design efficient DNA activators for you.

Targets of Biosensor Detection

  • Hg2+
  • Pb2+
  • Ag+
  • UO22+
  • Na+
  • Mg2+
  • K+
  • Cu2+

Applications

Food Safety Detection

Food Safety Detection

Disease Diagnosis

Disease Diagnosis

Environmental Monitoring

Environmental Monitoring

Process of Our Service

Process of Our Service

Advantages of Our Service

  • High Flexibility

Only the given gRNA sequence needs to be changed for different gene targets.

  • High Specificity

Single-base resolution detection of biosensors is fully possible.

  • High Sensitivity

Target analytes can be detected at concentrations as low as 10-18 mol/L.

CD BioSciences provides affordable, qualified and hassle-free metal ion biosensor development service to our global clients. We guarantee on-time delivery of results. We are available to you 24 hours a day, so please feel free to contact us.

References

  1. Xie, S.Y., et al. Advancing sensing technology with CRISPR: From the detection of nucleic acids to a broad range of analytes-A review. Analytica Chimica Acta. 2021, 338848.
  2. Chen, Y.J., et al. Applying CRISPR/Cas system as a signal enhancer for DNAzyme-based lead ion detection. Analytica Chimica Acta. 2021, (1192): 339356.
For research use only. Not intended for any clinical use.

Online Inquiry

Related Services

DeteCas™
  • SUITE 206, 17 Ramsey Road, Shirley, NY 11967, USA
  • 1-631-637-0420

Copyright © 2025 CD BioSciences. All rights reserved.