Immunological Diseases Protein Biomarker Detection Service

Immunological diseases are caused by abnormal functioning of the body's immune system causing the body to attack its own tissues. The detection of protein biomarker enables early diagnosis of immune diseases, which can be helpful in the treatment of patients.

Early detection of protein biomarkers is vital for immunological diseases diagnosis. As a professional analyte detection company, CD BioSciences is committed to providing efficient immunological diseases protein biomarker detection service.

Immunological Diseases Protein Biomarker Detection by CRIPSR-Cas System

The CRISPR-Cas system is derived from the adaptive immune system of microorganisms such as bacteria, archaea and fungi. As an up-and-coming technology in the field of biomolecules, it can be used both as a gene editing tool and as a sensitive and efficient biosensing tool. In the field of bioanalysis, the CRISPR-Cas system has the advantage of precise targeting and efficient cleavage of targeted or non-targeted nucleic acids, and thus can be used for protein biomarker detection for immunological disease diagnosis.

CRISPR-Cas9	CRISPR-Cas13
A new peptide display platform, PICASSO, has been developed using CRISPR-Cas9 technology. Using this platform, DNA molecules can be placed at specific locations on a surface, and each protein in the mixture then self-assembles to the corresponding DNA sequence, and the resulting DNA template protein microarrays allow you to quickly identify antibodies in clinical samples, or to identify any protein of interest to you.	Based on the signal amplification capability of the CRISPR-Cas system, researchers have developed an enzyme-linked immunosorbent assay using CRISPR-Cas13a as a signal amplification strategy, referred to as CLISA. The method allows for the detection of multiple immunological diseases protein biomarkers for disease diagnosis. The CARPID detection uses the CRISPR-Cas13d system to specifically tract a BASU biotin transferase fused to it to the vicinity of lncRNA in living cells, biotinylating the neighboring protein, which is subsequently separated using streptavidin-coupled magnetic beads, followed by label-free quantitative comparative analysis of the separated protein by mass spectrometry to confirm the binding of lncRNA proteins.

CRISPR-Cas9

CRISPR-Cas13

A new peptide display platform, PICASSO, has been developed using CRISPR-Cas9 technology. Using this platform, DNA molecules can be placed at specific locations on a surface, and each protein in the mixture then self-assembles to the corresponding DNA sequence, and the resulting DNA template protein microarrays allow you to quickly identify antibodies in clinical samples, or to identify any protein of interest to you.

Based on the signal amplification capability of the CRISPR-Cas system, researchers have developed an enzyme-linked immunosorbent assay using CRISPR-Cas13a as a signal amplification strategy, referred to as CLISA. The method allows for the detection of multiple immunological diseases protein biomarkers for disease diagnosis.

The CARPID detection uses the CRISPR-Cas13d system to specifically tract a BASU biotin transferase fused to it to the vicinity of lncRNA in living cells, biotinylating the neighboring protein, which is subsequently separated using streptavidin-coupled magnetic beads, followed by label-free quantitative comparative analysis of the separated protein by mass spectrometry to confirm the binding of lncRNA proteins.

Our Services

Utilizing the CRISPR-Cas system's ability to specifically identify and cleave nucleic acid sequences, CD BioSciences offer highly sensitive immunological diseases protein biomarker detection service. In addition, we also provide detection services for nucleic acids, metal ions and small molecules.

Our DeteCas™ platform can detect protein biomarker for different immunological diseases, including but not limited to the followings.

Type 1 Diabetes

Apo B (apolipoprotein B)
Alkaline Phosphatase

Systemic Lupus Erythematosus

ANuA (anti-nucleosome antibody)
CFB (complement factor B)
CFH (complement factor H)
rRNP (ribosomal protein)

Rheumatoid Arthritis

RF (rheumatoid factor)
Anti-CCP (anti-cyclic citrullinated peptide)
CRP (C-reactive protein)

Process of Our Service

Advantages of Our Service

High sensitivity for detection of low concentration substances.
High detection efficiency using an optimized CRISPR-Cas system.
Different detection solutions to meet different research needs.
Regular reporting of experimental progress.

CD BioSciences provides affordable, qualified and hassle-free Immunological Diseases Protein Biomarker Detection Service to our global clients. We guarantee on-time delivery of results. We are available to you 24 hours a day, so please feel free to contact us.

Reference

Karl, W.B., et al. CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays. Molecular Cell. 2021, 7(27): 3650-3658.

For research use only. Not intended for any clinical use.